ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Waraporn Sutthisa1 , Phatthraporn Nachai1 and Rattikan Yutthasin2
1Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai District, Mahasarakham Province, 44150, Thailand.
2Department of Agriculture, Office of Agricultural Research and Development, Region 3, Khon Kaen Province 40000, Thailand.
Article Number: 10426 | © The Author(s). 2025
J Pure Appl Microbiol. 2025;19(2):1410-1418. https://doi.org/10.22207/JPAM.19.2.44
Received: 28 March 2025 | Accepted: 15 May 2025 | Published online: 02 June 2025
Issue online: June 2025
Abstract

Mango anthracnose, caused by Colletotrichum gloeosporioides, is a significant disease affecting mango production. This study aimed to develop and evaluate a bio-formulation of Serratia nematodiphila GCSR38 for controlling mango anthracnose. The antagonistic activity of S. nematodiphila GCSR38 was tested using the dual culture method, achieving a 40.21% inhibition rate against C. gloeosporioides. Three powder formulations were developed using a low-heat drying process, differing in their composition of corn starch, talcum, calcium carbonate, and carboxymethyl cellulose (CMC). The formulations exhibited varying physical characteristics, water solubility, and moisture stability, with pH values ranging from 7.90 to 8.00. The survival of S. nematodiphila GCSR38 in the powder formulations remained substantial over 120 days, with final bacterial counts between 2.50 × 107 and 5.10 × 107 CFU/g. Efficacy testing on mango leaves showed that Bio-Formulation 3 provided the highest inhibition of C. gloeosporioides, achieving 51.00% inhibition in preventive application and 48.30% in curative application. These results were comparable to the chemical fungicide Carbendazim (56.11% and 49.31%, respectively), with no significant differences observed. Preventive application proved more effective than curative treatment. Overall, S. nematodiphila GCSR38 shows promise as a biocontrol agent for mango anthracnose, offering an eco-friendly alternative to chemical fungicides.

Keywords

Serratia nematodiphila GCSR38, Mango Anthracnose, Powder Bio-product, Preventive Application, Curative Application

Article Metrics

Article View: 190

Share This Article

© The Author(s) 2025. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.