ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ahmed Salah1, Naemia M. Yousef2, Abd El-Latif Hesham3 , Ingy M. El-Hefny4 and Asmaa M.M. Mawad2,5,6
1Department of Environmental Science and Pollution Treatment, Faculty of Sugar and Integrated Industries Technology, Assiut University, Assiut, Egypt.
2Department of Botany and Microbiology, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
3Department of Genetics, Faculty of Agriculture, Beni-Suef University, Beni-Suef 62511, Egypt.
4Faculty of Biotechnology, Modern Sciences and Arts University, Giza, Egypt.
5Department of Biology, College of Science, Taibah University, Madinah, Saudi Arabia.
6Health and Life Research Center, Taibah University, Madinah – 42353, Saudi Arabia.
Article Number: 9961 | © The Author(s). 2025
J Pure Appl Microbiol. 2025;19(2):1114-1123. https://doi.org/10.22207/JPAM.19.2.13
Received: 04 October 2024 | Accepted: 02 March 2025 | Published online: 07 May 2025
Issue online: June 2025
Abstract

Microorganisms exhibit a complex relationship with heavy metals. While some of these metals are essential for microbial growth and function, others can be detrimental at elevated concentrations, inhibiting or even killing the organisms. In this study, free living diazotrophic bacteria were isolated from contaminated sites. The bacteria were investigated to resist different concentrations of Zn (II); 5-60 mg/L. The results revealed that the bacterial isolate was identified as Bacillus subtilis AUMC-B492 based on 16S rRNA gene sequencing. The bacteria were diazotrophic in nutrition and can fix atmospheric nitrogen for growth and replication in nitrogen free media. B. subtilis AUMC-B492 removed 30% of Zn (II) when initial concentration was 5 mg/L within 48 h in nitrogen free media. It could tolerate up to 100 mg/L of Zn (II) within 24 h. Therefore, strain AUMC-B492 could be used as a promising tool for bioremediation of heavy metals as well as holding potential for agricultural applications.

Keywords

Bacteria, Bacillus, Heavy Metal, Resistance, Zinc Ions

Article Metrics

Article View: 52

Share This Article

© The Author(s) 2025. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.