Hesperetin, a natural flavonoid, has been investigated for its potential therapeutic properties. In this study, we delve into its antimicrobial and antiviral activities against clinically relevant bacterial and fungal strains. Our investigation unveiled substantial antimicrobial and antioxidant properties of hesperetin against a diverse array of pathogens, encompassing both Gram-positive as well as Gram-negative bacteria. Furthermore, notable antifungal activities were observed, particularly against resistant fungal strains. The findings from our study underscore the potential of hesperetin as a promising candidate for the development of broad-spectrum antimicrobial agents. Overall, hesperetin exhibits versatility with implications for combating infectious diseases. These insights pave the way for further exploration of hesperetin’s therapeutic applications and its potential utility in addressing the challenges posed by antimicrobial resistance.