Lignocellulosic residues, including rice stubble, carboxymethyl cellulose (CMC), xylan, and lignin, were evaluated as substrates for cultivating lignocellulolytic fungi in submerged fermentation at 30 °C over 15 days. Rice stubble, accounting for 40-60% of rice plant biomass and containing 42.14% cellulose, 22.08% hemicellulose, and 11.98% lignin, was explored as a renewable resource for energy and biochemical production. Four fungal strains-Penicillium oxalicum (F1), Talaromyces pinophilus (F12), Penicillium griseofulvum (F22), and Trichoderma reesei (F26) were evaluated for their lignocellulolytic enzyme production potential. Enzyme assays conducted at 3-day intervals revealed maximal production of CMCase (63.42-88.26 U/mL), FPase (46.01-80.66 U/mL), xylanase (1146.10-1640.52 U/mL), lignin peroxidase (0.192-0.287 U/mL), and laccase (0.193-0.434 U/mL). ITS (internal transcribed spacer) sequencing confirmed the fungal strain identities. These findings highlight the potential of the tested fungal strains for hydrolytic stubble production and lignocellulose degradation, positioning rice straw as a cost-effective carbon source for biotechnological applications in biorefineries.