ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mrigakshi Gogoi1, Pankaj Chetia2, Raj Kumar Pegu3 and Jayanti Datta Roy4
1Department of Microbiology, Assam Don Bosco University, Kamarkuchi, Tepesia Gardens, Sonapur, Assam, India.
2Department of Life Sciences, Dibrugarh University, Assam, India.
3Department of Botany, Assam Don Bosco University, Kamarkuchi, Tepesia Gardens, Sonapur, Assam, India.
4Department of Biosciences, Assam Don Bosco University, Kamarkuchi, Tepesia Gardens, Sonapur, Assam, India.
Article Number: 9779 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(4):2791-2805. https://doi.org/10.22207/JPAM.18.4.52
Received: 01 August 2024 | Accepted: 25 October 2024 | Published online: 27 November 2024
Issue online: December 2024
Abstract

Staphylococcus aureus is the leading cause of milk-borne disease in animals and humans worldwide, and it is often contaminated by enterotoxigenic and antimicrobial-resistant S. aureus strains. The current research work was intended to identify the prevalence of S. aureus from samples of bovine milk from various dairy farms and local vendors of Kamrup Metro District, Assam, India, by phenotypic and genotypic identification along with antibiotic resistance profiling. The conventional aseptic methods were implemented for S. aureus isolation from milk in Baird Parker Agar, supplemented with egg yolk and potassium tellurite. Further, the isolates confirmation was carried out using the automated VITEK system and amplification of the S. aureus specific nuc gene by PCR. Antibiotic susceptibility profiling for variety of 16 antibiotics was obtained through the conventional disc diffusion method. Eighty-five presumptive isolates with jet-black colonies with a white halo on Baird Parker Agar were selected. Thirty-eight isolates were eventually confirmed as S. aureus by the automated method and the detection of nuc gene. Antibiotic profiling revealed about 60.52% of the isolates to be multidrug resistant and 55.26a ± 0.01 mm resistant against Kanamycin. The statistical analysis data expressed correlation between Penicillin G and Ampicillin with 42.10b ± 0.01 mm and correlation among Tetracycline, Methicillin and Streptomycin with 10.52h ± 0.01 mm, respectively. Resistance against Kanamycin, Trimethoprim, Cloxacillin, and Nalidixic acid is concerning as such a resistant pattern has not been extensively reported in bovine milk samples in India, which could indicate the possible emergence of MDR S. aureus strains in the study area.

Keywords

Bovine Milk Staphylococcus aureus, nuc Gene, Antimicrobial Resistance

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.