ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Abeer Al Mousa1, Munirah Aldayel1, Marwa A.M. Genena1,2, Zeinab A. El-Moaty1 and Ashraf Khalifa1,3
1Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia.
2Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Egypt.
3Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Article Number: 9133 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1358-1371. https://doi.org/10.22207/JPAM.18.2.59
Received: 15 November 2023 | Accepted: 21 May 2024 | Published online: 02 June 2024
Issue online: June 2024
Abstract

This study aimed to investigate bacterial diversity in Al-Asfar Lake, Al Ahsa Oasis, Saudi Arabia, based on a polyphasic approach. Water samples were collected and divided into two parts. For the culture-dependent approach, different media such as Tryptic Soy Agar (TSA), and Blood Agar (BA) were used. The obtained 29 isolates were diverse with respect to phenotypic characteristics revealed by VITEK. 18 isolates were selected for 16S rRNA sequencing based on the initial screening by VITEK. Since some types of bacteria do not grow on media, the DNA in the second part was isolated directly and subjected to metagenomic analysis. VITEK disclosed a total of 19 species belonging to 3 phyla: Pseudomonadota, Actinomycetota, and Bacillota, while the 16S rRNA sequencing revealed 12 species that could be amplified and sequenced. Metagenomic analysis exhibited variation in the relative abundance of 13 phyla, 18 classes, 20 families, 16 genera, and 24 species. Four dominant phyla are represented, including Firmicutes, Cyanobacteria, Bacteroidota, and Proteobacteria, respectively. Furthermore, functional gene screening revealed 33 functional categories including the metabolism of cofactors, vitamins, and xenobiotic biodegradation. This study affords insights into the bacterial diversity and fundamental biogeochemical processes in the lake and paves the way for the potential exploitation of microbial wealth in biotechnological applications.

Keywords

Al-Asfar Lake, Bacterial Diversity, Al-Ahsa, VITEK, 16S rRNA Sequencing, Metagenomic Analysis

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