ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
M. Indira, G. Bhuvaneshwari , L. Premkumar and P. Neelusree
Department of Microbiology, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Thandalam, Kanchipuram, Chennai, Tamil Nadu, India.
Article Number: 9209 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1297-1304. https://doi.org/10.22207/JPAM.18.2.50
Received: 27 December 2023 | Accepted: 10 May 2024 | Published online: 02 June 2024
Issue online: June 2024
Abstract

Garlic contains various components, such as allicin, ajoenes, and allyl sulfides, which are organosulfur compounds. It exhibits antimicrobial activities in all forms, including garlic powder, garlic extract, and garlic oil. Additionally, it exerts anti-inflammatory, antibacterial, antibiofilm, and antifungal effects. The drastic increase in drug-resistant organisms worldwide, especially methicillin-resistant Staphylococcus aureus (MRSA) strains, has led to treatment failure in various infectious diseases. To overcome this issue, we aimed to assess the antibacterial activity of garlic extract against MRSA and explore its action mechanism against penicillin-binding proteins (PBPs) in this study. Mueller–Hinton agar was used for the agar well diffusion test. Moreover, bactericidal activity was determined using the Mueller–Hinton agar and spot tests. Molecular docking was performed using AUTODOCK to evaluate the PBP-binding ability of allicin. A wide zone of inhibition was observed for MRSA, and molecular docking revealed a strong interaction between PBP3 of S. aureus and allicin. Overall, our findings revealed the antibacterial activity of garlic extract against MRSA, a prevalent gram-positive coccus causing healthcare-associated infections. In addition to its affordability, the beneficial effects of garlic suggest its therapeutic potential for MRSA treatment in combination with cefoxitin.

Keywords

Allicin, Antibacterial Activity, Staphylococcus aureus, Cefoxitin

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.