ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Abhishek Kumar1, Ruchi Sankhwar1, Shilpi Yadav2 and Ravi Kr. Gupta1
1Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Lucknow, Uttar Pradesh, India.
2Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Lucknow, Uttar Pradesh, India.
Article Number: 9317 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1196-1208.
Received: 21 February 2024 | Accepted: 01 May 2024 | Published online: 27 May 2024
Issue online: June 2024

Pseudomonas aeruginosa is capable of producing biofilms on various surfaces, medical implants and burn wounds which inhibit the action of antimicrobial agents. This study was undertaken for the screening, identification and purification of a natural antimicrobial compound from the hospital waste sites against P. aeruginosa. The partial purification of the antimicrobial agent was performed with ethyl acetate from the culture supernatant of the isolate and further purified by HPLC. For the characterization of the active compound, we have used Fourier Transform Infrared Spectroscopy (FT-IR) and Electrospray Ionization mass spectrometry (ESI-MS) analysis. The purified compound was tested for anti-biofilm activity in vitro against P. aeruginosa and on various surfaces such as plastic, glass, and steel. The potent isolate was confirmed as Bacillus licheniformis strain RG1002. The HPLC purified compound was characterized as lichenysin using FTIR and ESI-MS analysis. This study identifies and characterize the potent antimicrobial agent against other important human pathogens such as Staphylococcus aureus, Salmonella typhi, Shigella flexneri and Escherichia coli. Purified lichenysin showed strong inhibitory effect against P. aeruginosa and other important pathogens and inhibits the biofilm formation of P. aeruginosa in vitro on various types of surfaces. The current study suggests the application of lichenysin as a protective coating on various indwelling medical devices and catheters to prevent the biofilm formation by P. aeruginosa.


Antimicrobial Agents, Biofilms, Pseudomonas aeruginosa, Lichenysin, Bacillus licheniformis RG1002

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.