ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Janice Ravi Kumar1, Dayanandan Anandan1 and Viswanathan Kaliyaperumal2
1Molecular and Nanomedicine Research Unit, Centre For Nano Science and Nanotechnology (CNST), Sathyabama Institute of Science and Technology, Chennai, India.
2Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India.
Article Number: 9211 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1137-1150. https://doi.org/10.22207/JPAM.18.2.33
Received: 28 December 2023 | Accepted: 27 March 2024 | Published online: 27 May 2024
Issue online: June 2024
Abstract

The lipid-producing gram-positive bacteria were isolated from soil and it was identified as Streptomyces fradiae JJ1 MK733985.1. Bacterial lipid synthesis was explored using rice washed water through the fermentation research. The exponential growth phase was reached on the fifth day of fermentation, according to the results. The ability of Streptomyces fradiae to produce microbial oil was compared to that of International Streptomyces Project-2 Medium (ISP2) medium. The results showed that RWW culture medium yielded 85.4% fatty acids and 60.3 g/L biomass, while ISP2 medium yielded 83.2% wt fatty acids and 59.5 g/L biomass. The microbial oil was transesterified into Fatty acid methyl esters (FAME), and its characteristics were studied using American Society for Testing Materials (ASTM) standards. The specific gravity was 0.864, the density was 0.863, the gross heating value was 42498 kj/kg, the cetane number was 37, and the acid value was 0.46. Streptomyces fradiae grew with an ISP2 density of 0.835, specific gravity of 0.845, gross heating value of 42326 kj/kg, and acid value of 0.43. According to the findings, Streptomyces fradiae isolated from soil can grow in RWW medium for the first time and provide the same yield as commercial media while being significantly less expensive.

Keywords

Biodiesel, Streptomyces fradiae, Rice Washed Water, Fermentation, Soil Bacteria

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