ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S.L. Varsha1, Arun K. Shettar2, Joy H. Hoskeri3 and A.B. Vedamurthy1
1Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.
2Division of Pre-clinical Research and Drug Development, Cytxon Biosolutions Pvt. Ltd., Hubli, Karnataka, India.
3Department of Bioinformatics and Biotechnology, Karnataka State Akkamahadevi Women’s University, Vijayapura, Karnataka, India.
Article Number: 9294 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1126-1136. https://doi.org/10.22207/JPAM.18.2.32
Received: 05 February 2024 | Accepted: 25 April 2024 | Published online: 27 May 2024
Issue online: June 2024
Abstract

Some microorganisms known as endophytes live in symbiotic relationships in the living tissues of plants without posing a health risk. As a result, they synthesize many metabolites which are helpful for the plants in many ways. So, these metabolites are known to exhibit many biological properties like antioxidant, antidiabetic anti-inflammatory, etc. Currently, many drugs are used to control inflammatory diseases like arthritis and, irritable bowel disease; however, they pose a lot of side effects. The present study was taken up to explore the anti-inflammatory properties along with the phytochemicals present, its quantification, and other in vitro biological activities of the less reported Aspergillus melleus, an endophytic fungus, isolated from Premna serratifolia L., a medicinal plant. The results of the investigation demonstrated the presence of alkaloids, phenols, terpenoids, flavonoids, tannins, cardiac glycosides and amino acids in the methanolic extract of endophytic fungus. It yielded 25.28 µg GAE/g and 19.465 µg GAE/g of total phenolic and flavonoid content, respectively. The results of anti-inflammatory activity showed 84.69+0.82% protein inhibition by BSA and also showed IC50 values of 68.53 µg/mL and 43.34 µg/mL for COX1 and COX 2, respectively. It exhibited 63.91+0.08% of radical scavenging activity by DPPH. The IC50 values of 181.41 µg/mL and 190.62 µg/mL were found for the in vitro antidiabetic activity. This study shows that the endophytic fungus A. melleus has exhibited considerably good results with respect to its in vitro biological activities. Yet, there is a scope for future researchers to isolate the bioactive metabolites to explore for future needs.

Keywords

Aspergillus melleus, Anti-inflammatory Activity, COX-1, COX-2, Antidiabetic Activity

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.