ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sabrine Raffa1, Adel Ayari2 and Ines Guehria3
1Laboratory of Sciences and Technology of Water and Environment (LSTWE), University of Souk-Ahras, PO box 1553, Souk Ahras, 41000, Algeria.
2Laboratory of Organic Chemistry and Interdisciplinary (LOCI), University of Souk-Ahras, PO box 1553, Souk Ahras, 41000, Algeria.
3Laboratory of Biochemistry and Biotechnology (LBB), Faculty of Sciences of Tunis, University of El Manar, University Campus El-Manar, El-Manar, 2092, Tunisia.
Article Number: 9233 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1093-1102.
Received: 11 January 2024 | Accepted: 01 April 2024 | Published online: 27 May 2024
Issue online: June 2024

In this study, an Actinomycetota Streptomyces mediolani strain CF51DZ was isolated from a beetle Protaetia morio collected in a cork oak forest in northeast Algeria, to extract, purify and characterize the antibacterial compound. The strain was isolated in specific ISP culture media, identified via morphological, biochemical, physiological and molecular characteristics and then studied its antibacterial activities against pathogenic bacteria using the well diffusion technique. The extraction, purification and characterization of the bioactive compound was carried out using chromatographic and spectroscopic methods. Morphological, physiological, and biochemical properties, as well as the 16S ribosomal DNA sequence of the strain, were found close to those of Streptomyces mediolani. The isolated strain produced an active compound having potent antibacterial properties against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. This compound was isolated and purified with thin layer chromatography and high-performance liquid chromatography, and chemically identified by mass spectroscopy and NMR spectroscopy. In addition, the chemical structure elucidation of the antibacterial metabolite by APCI-MS analysis revealed a peak at m/z 375 [M+H]+, a molecular formula of C19H18O8. This compound was identified as methyl rosarinate: 3-(3,4-dihydroxyphenyl)-1-methoxy-1-oxopropan-2-yl (E)-3-(3,4-dihydroxyphenyl)acrylate based on the IUPAC nomenclature. Moreover, the antibacterial agent was purified from Streptomyces mediolani strain CF51DZ that was for the first time, in Algeria, isolated from the beetle Protaetia morio. This supports further characterization of this promising chemical to ensure the best use for antibacterial applications.


Actinomycete, Beetle Protaetia morio, Streptomyces mediolani strain CF51DZ, 16S Ribosomal DNA Sequence, Antibacterial Activity, Chemical Structure

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.