ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nuzhat Maqbool Peer1, Vinod Joshi1 , Annette Angel1, Bennet Angel1, Vikram Singh Chauhan2, Shareef Mohammed Buvvaji1, Poorna Khaneja1, Ambreen Shafaat Khan1, Shilpa Barthwal1, Neha Singh1, Bhawna Sharma1, Ramesh Joshi3, Monika Dheer1, Khushbu Kumari1, Aarya Chitransh1 and Reshu Chauhan1
1Centre of Excellence in Virology & Immunology, Sharda University, Greater Noida, Uttar Pradesh, India.
2Department of Surgery, Sharda School of Medical Sciences & Research, Sharda University, Greater Noida, Uttar Pradesh, India.
3Sharda School of Agricultural Sciences, Sharda University, Greater Noida, Uttar Pradesh, India.
Article Number: 9202 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1057-1063. https://doi.org/10.22207/JPAM.18.2.21
Received: 23 December 2023 | Accepted: 23 April 2024 | Published online: 15 May 2024
Issue online: June 2024
Abstract

Hepatitis C virus infection is one of the main causes of liver disease that can lead to liver cirrhosis and finally to the chronic development of Hepatocellular Carcinoma (HCC). With 8 different HCV genotypes circulating, it becomes necessary to characterize the pathogenesis caused by each one of the HCV genotypes infecting population of a particular setting. Present study aims to identify the circulating Hepatitis C virus (HCV) genotypes in the different areas of State of Uttar Pradesh, India and understand their clinical etiology and related co-morbidities. The patients reporting to Sharda Hospital, Uttar Pradesh, India who were diagnosed positive for HCV were included in the study. The informed consent was obtained and then blood was drawn. In-house primers, for all the 8 genotypes were prepared and RT-PCR was performed. The amplified product was subjected to Agarose gel electrophoresis to identify the genotypes present in individual samples. Total 30 serum samples were taken. Of these, 21 (70%) showed presence of multiple genotypes, while 7 (23.33%) showed positivity for single genotype and two did not show any bands. Presence of multiple genotypes in individual patient has been reported for the first time. The pathological consequences of multiple genotypes within one host need to be studied further in terms of increased risk of developing a chronic liver disease. Further studies are being undertaken to study association of specific HCV genotype and multiple genotypes, Core, NS3 and NS5 viral proteins with the disease progression to develop predictors of liver cirrhosis and HCC.

Keywords

Genotypes, Hepatitis C virus, Hepatocellular carcinoma, India

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.