ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Prajakta S. Pawar, Satyajeet K. Pawar , Satish R. Patil, Harsha V. Patil and Priyanka M. Mane
Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth “Deemed to be University”, Karad, Malkapur (Dist. Satara) Maharashtra, India.
Article Number: 8998 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1025-1031. https://doi.org/10.22207/JPAM.18.2.17
Received: 15 September 2023 | Accepted: 28 March 2024 | Published online: 02 May 2024
Issue online: June 2024
Abstract

Klebsiella pneumoniae is one of the most common bacteria among all biofilm-producing as well as the beta-lactamase producing strains, which is responsible for multi-drug resistance. For better therapeutic applications, it is important to detect the biofilm production by Klebsiella pneumoniae and their antibiogram along with the ability to produce ESBL, MBL, and AmpC β-lactamases. The aim of the study was to determine the prevalence of biofilm formation and ESBL, MBL, AmpC β-lactamase phenotypes in K. pneumoniae as well as the antibiogram of all (biofilm and MBL-producing and non-producing) isolates of K. pneumoniae. Isolates of K. pneumoniae were tested for biofilm formation by the Congo-red agar method. ESBL, MBL, and AmpC β-lactamase detection were done by both screening and confirmatory tests as per CLSI guidelines. The antibiogram was obtained by the Kirby-Bauer disc diffusion method. Among the total 100 isolates of K. pneumoniae, 40% were biofilm-producing. Most of them were from urine specimens. Out of biofilm-producing isolates, ESBL – 28%, MBL- 47% and AmpC β-lactamase- 25.8% producers were observed. K. pneumoniae isolates were seen to have maximum resistance to ceftazidime and maximum sensitivity to nitrofurantoin. Study findings suggest the importance of assessment of biofilm formation for better treatment. The scenario further worsens if such biofilm-producing isolates are also MBL-positive leading to limited therapeutic options.

Keywords

Biofilm Formation, Extended Spectrum Beta-lactamases, Metallo-Beta-Lactamases, Ampicillinase Cephalosporinase

Article Metrics

Article View: 93

Share This Article

© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.