ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ramya Rengaraj1 , Natarajan Muninathan2, Sivaranjini Alagiri3 and Arumugam Suresh2
1Department of Microbiology, Melmaruvathur Adhiparasakthi Institute of Medical Sciences and Research, Melmaruvathur, Tamil Nadu, India.
2Central Research Laboratory, Meenakshi Medical College Hospital and Research Institute, Kanchipuram, Tamil Nadu, India.
3Department of Microbiology, Sri Lakshmi Narayana Institute of Medical Sciences, Puducherry, India.
Article Number: 9214 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):1004-1012. https://doi.org/10.22207/JPAM.18.2.15
Received: 22 December 2023 | Accepted: 02 April 2024 | Published online: 02 May 2024
Issue online: June 2024
Abstract

Pseudomonas aeruginosa, a Gram-negative bacterium, presents a substantial challenge in healthcare due to its adaptability and resistance. This study delves into its genotypic characteristics, focusing on ESBL and MBL genes. The prevalence of P. aeruginosa in nosocomial infections, the research aims to decipher resistance mechanisms crucial for tailored interventions. The study includes 170 non-repetitive clinical samples with protocols. Antibiotic susceptibility testing reveals diverse resistance patterns. Molecular detection of ESBL and MBL genes involves DNA isolation, PCR amplification, and gel electrophoresis. The study examined 170 P. aeruginosa samples, revealing gender-specific variations with 65.91% male and 34.09% female isolates. Antimicrobial testing displayed resistance in Ceftazidime (59%) and Ciprofloxacin (48%), while Ticarcillin-clavulanic acid showed promising sensitivity (58%). Molecular identification unveiled diverse resistance genes across sample types, emphasizing genetic complexity. The study underscores the urgency for targeted therapeutic interventions and novel antimicrobial strategies against P. aeruginosa infections. As antimicrobial resistance complexities persist, this research guides efforts toward a profound understanding of clinical interventions and strategic antimicrobial management.

Keywords

Pseudomonas aeruginosa, ESBL, Multidrug Resistance (MDR), Antimicrobial Resistance

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.