ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Khuzama Alarfaj1, Ahmad Almatroudi1, Faris Alrumaihi1, Arshad Hussain Rahmani1, Amjad Ali Khan2, Hamid G. Mohamed2,
Masood Alam Khan2, Shaden Abdullah Alalawi3, Nada Alkhorayef4and Khaled Allemailem1
1Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
2Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
3Department of Medicine, College of Medicine, Qassim University, Buraydah, Saudi Arabia.
4Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Al-Quwayiyah, Shaqra University, Riyadh, Saudi Arabia.
Article Number: 9138 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(2):900-906. https://doi.org/10.22207/JPAM.18.2.03
Received: 16 November 2023 | Accepted: 04 March 2024 | Published online: 29 March 2024
Issue online: June 2024
Abstract

The escalation of environmental pathogenic microorganisms and disregard of public hygiene practices have resulted in the emergence of various skin infections within communities. Recent investigations have proposed that diverse snail mucus compositions may possess antimicrobial properties. Therefore, it is imperative to conduct further research to elucidate specific antibacterial characteristics inherent in the mucus of White Garden snail (Theba pisana). This study aimed to evaluate antibacterial activity of Theba pisana mucus extract against selected ATCC bacterial strains being Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and MRSA. The assessment involved comparing antimicrobial activity of the mucus extract to various broad-spectrum antibiotics. The susceptibility of bacterial isolates to snail mucus secretions was assessed using agar well diffusion method on Muller Hilton Agar plates. After incubation at 37°C for 24 hours, various concentrations of snail slime including 100%, 75%, 50%, and 25% were applied. The findings reported that E. faecalis exhibited highest activity, with zone of inhibition measuring 24 mm, followed by K. pneumonia and S. aureus (16 mm), MRSA (14 mm), and E. coli (12 mm). However, at concentration of 25%, mucus extract exhibited effect only against E. faecalis. Moreover, the antimicrobial activity of several tested antibiotics demonstrated similarity to that of the mucus extract. Therefore, it revealed that secretions of T. pisana mucus may possess the potential to act as a source of antibacterial agents. This may become as an alternative agent to costly synthetic antibacterial compounds. However, further studies are required to exploit the mucus secretion in addressing the issue of antibiotic resistance.

Keywords

White Garden Snail, Theba pisana, Bacteria, Antibacterial Activity, Bacterial Resistance

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.