ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S. Amitha Banu1, Shubham Saini2, Khan Sharun1 , Merlin Mamachan1,Sonu S. Nair3, Abhijit M. Pawde1, Kuldeep Dhama4 , Laith Abualigah5-11 and Swapan Kumar Maiti1
1Division of Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
2Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
3Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
4Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
5Artificial Intelligence and Sensing Technologies (AIST) Research Center, University of Tabuk, Tabuk 71491, Saudi Arabia.
6Hourani Center for Applied Scientific Research, Al-Ahliyya Amman University, Amman 19328, Jordan.
7MEU Research Unit, Middle East University, Amman 11831, Jordan.
8Department of Electrical and Computer Engineering, Lebanese American University, Byblos 13-5053, Lebanon.
9School of Computer Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia.
10School of Engineering and Technology, Sunway University Malaysia, Petaling Jaya 27500, Malaysia.
11Applied Science Research Center, Applied Science Private University, Amman 11931, Jordan.
Article Number: 9254 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):653-661.
Received: 16 January 2024 | Accepted: 08 February 2024 | Published online: 02 March 2024
Issue online: March 2024

This study aimed to assess and manage bacterial contamination in multiple batches of mesenchymal stem cell (MSC) cultures derived from rabbit bone marrow. Routine visual inspection and microscopic examination were employed for the detection of the contaminated cultures. The contaminated cultures were inoculated on Nutrient agar and multiple isolated colonies were subjected to Gram staining and biochemical characterization. Further, molecular identification of the bacterial isolates was performed using polymerase chain reaction. The determination of antibiotic susceptibility patterns was conducted using the Kirby-Bauer disc diffusion method. Among the 351 mesenchymal stem cell culture (SCC) flasks monitored, only 1.42% were found to be contaminated. Based on the phenotypic and biochemical characterization, the major bacterial contaminants were identified as Staphylococcus aureus, Bacillus spp., and Escherichia coli infiltrating during various stages of cell processing. Antibiotic susceptibility patterns revealed varying responses among isolates, crucial for effective antimicrobial strategies and maintaining aseptic conditions in SCCs. The study emphasizes the importance of regular monitoring to maintain sterile environments, validate culture quality, and uphold safety standards. The findings indicate the need to establish stringent quality control measures, crucial for the successful translation of MSC research into clinical applications. The research advocates for continuous monitoring, adherence to SOPs, and further investigations into preventive strategies for ensuring the safety and efficacy of MSC-based therapies.


Cell Culture Contamination, Stem Cell Culture, Mesenchymal Stem Cells, Bacterial Contaminants

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.