Actinomycetes are the potential producers of secondary metabolites of vivid applications; they are isolated from almost all the sources both terrestrial and aquatic habitats. Actinomycetes are a group of Gram-positive bacteria known for their filamentous structure and ability to produce a diverse array of bioactive compounds. These bioactive compounds include antibiotics, antifungals, antivirals, anticancer agents, immunosuppressants, and enzymes. Actinomycetes have been a major source of these bioactive compounds and have played a significant role in the development of many therapeutic drugs. Actinomycetes, which are isolated from practically all sources in both terrestrial and aquatic ecosystems, have the potential to create secondary metabolites with diverse uses. A class of Gram-positive bacteria called actinomycetes is distinguished by its filamentous structure and capacity to manufacture a wide range of bioactive substances. Antibiotics, antifungals, antivirals, cancer preventatives, immunosuppressants, and enzymes are a few examples of these bioactive substances. These bioactive substances have primarily come from actinomycetes, which have also contributed significantly to the creation of several medicinal medications. However, actinomycetes isolation and cultivation can be challenging due to their slow growth rate and complex nutritional requirements. In order to isolate and cultivate actinomycetes, several pre-treatment methods and media can be employed.