ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
M.S. Vineetha1, Nayef Abdulaziz Aldabaan2, Sunil S. More1 ,
Mater H. Mahnashi3, Ibrahim Ahmed Shaikh2, Mohammad Shahzad Samdani4, Rashmi Swami1, Anirudh Yadav1, N. Rohith1, J. Bhavya1, Basheerahmed Abdulaziz Mannasaheb5, Mamdouh Saleh Alharbi5,
Aejaz Abdullatif Khan6, Salah Eldeen Dafalla7,8 and S.M. Shakeel Iqubal6
1School of Basic and Applied Sciences, Dayananda Sagar University, Bangalore, Karnataka, India.
2Department of Pharmacology, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia.
3Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia.
4College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
5Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Saudi Arabia.
6Department of General Science, Ibn Sina National College for Medical Studies, Jeddah 21418, Saudi Arabia.
7Department of Human Anatomy, Ibn Sina National College for Medical Studies, Jeddah, 21418, Saudi Arabia.
8Eastern Sudan College for Medical Sciences and Technology, Port Sudan, J6C8+QX7, Sudan.
Article Number: 8937 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):593-604. https://doi.org/10.22207/JPAM.18.1.43
Received: 24 August 2023 | Accepted: 07 February 2024 | Published online: 29 February 2024
Issue online: March 2024
Abstract

L-glutaminase is a unique enzyme with catalytic activity and the ability to modulate glutamine levels, making it a valuable enzyme with numerous potential applications. L-glutaminase triggers a distinctive reaction by converting L-glutamine into glutamic acid while releasing ammonia concurrently. This enzymatic process holds potential applications across diverse industries, notably in food and pharmaceuticals. The primary objective of the present research was to identify and isolate a fungal strain proficient in L-glutaminase production from soil found in maritime environments. The physical and nutritional conditions were optimized for maximum synthesis of L-glutaminase under solid state fermentation (SSF) and submerged fermentation conditions (SmF). The isolated organism was identified as Fusarium solani-melongenae strain CRI 24 by morphological and 18S rRNA analysis. The optimum carbon source under SmF and SSF was found to be starch (0.2% w/v). Wheat bran as solid substrate among others showed optimum enzyme activity. On the seventh day of incubation, at pH 8.0 and 0.7% L-glutamine concentration under SSF and SmF, the highest enzyme activity was detected. The greatest enzyme activity in SSF was seen at a moisture content of 10%. Fusarium solani-melongenae species produced the enzyme under optimal conditions and 4.20 and 4.73-fold increase (from 0.8 U/mL to 3.61 U/mL and from 0.781 U/mL to 3.69 U/mL) was achieved after optimization in submerged and in solid state fermentation, respectively. The selective isolation and optimization processes described in this work are a promising technique for the industrial production of L-glutaminase and can be applied in the pharmaceutical and food industries.

Keywords

L-Glutaminase, Fusarium solani-melongenae, Solid State Fermentation, Submerged Fermentation

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.