ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Eman A. El-Masry1,2 , Mohamed Samir3,4 and Ahmed E. Taha1,5
1Microbiology and Immunology Unit, Department of Pathology, College of Medicine, Jouf University, Sakaka 72388, Saudi Arabia.
2Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
3Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Egypt.
4The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 0NF, United Kingdom.
5Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
Article Number: 9131 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):568-583. https://doi.org/10.22207/JPAM.18.1.40
Received: 14 November 2023 | Accepted: 09 February 2024 | Published online: 29 February 2024
Issue online: March 2024
Abstract

Infection with tuberculosis (TB) has been a leading cause of death worldwide. The accurate detection of Mycobacterium tuberculosis (M. tuberculosis) is integral for the prevention and control programs for TB disease. Host-encoded microRNAs (miRNAs) are known to be triggered upon TB infection, raising possibilities of their utility as biomarkers. Here, we investigated the value of miRNAs in pointing active TB (ATB) and predicting the progression from latent TB (LTB) to ATB infection. We then constructed signaling pathways predicted to be involved in TB disease pathobiology. Our analyses identified miR-21-5p, miR-29a-3p and miR-361-5p as being down regulated in ATB and upregulated in LTB compared to healthy subjects with an area under the curve (AUC) of > 0.9, indicating potentially promising biomarker. Pathways related to estrogen signaling, TNF and apoptosis signaling were enriched targets for those miRNAs. This study provides evidence for the significance of miRNA as diagnostic and prognostic markers for ATB and LTB infections in humans. These results could be extrapolated and validated using future large-scale studies.

Keywords

Pulmonary Tuberculosis, MicroRNAs, Small Non-coding RNAs, Biomarkers

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.