ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Haseena Muhammad1 , Najla Ali Alburae1 , Mohamed Abdel Salam2, Munair Badshah3, Taous Khan4 and Salah E.M. Abo-Aba1
1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
2Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
3Mukabbir College of Pharmacy, Gujrat, Punjab, Pakistan.
4Department of Pharmacy, COMSATS University Islamabad Abbottabad Campus, Abbottabad, Pakistan.
Article Number: 9125 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):483-499. https://doi.org/10.22207/JPAM.18.1.32
Received: 11 November 2023 | Accepted: 09 January 2024 | Published online: 28 February 2024
Issue online: March 2024
Abstract

Bacterial cellulose (BC) stands out as a prominent biopolymer of global importance, distinguished by its unique advantages over plant-derived cellulose. Strains such as Acetobacter xylinum, renowned for their proficient BC production, draw considerable attention in both commercial and biomedical areas. This research aimed to selectively isolate cellulose-producing bacteria with enhanced efficiency from a variety of fruit samples utilizing a cost-effective methodology. A total of 60 fruit samples were selected, and the assessment focused on 17 strains derived from rotten banana, red apple, green apple, and pineapple samples. The evaluation encompassed an examination of bacteriological traits and cellulose synthesis, with subsequent identification of strains achieved through DNA extraction and 16S rRNA PCR analysis. The experimental findings reveal cellulose-producing strains, including model A. xylinum (KCCM 40407) obtained from the Pharmacy lab of COMSATS University Islamabad Abbottabad Campus, Pakistan, designated as number 2, serving as a control. Notably, strains isolated from deteriorated fruits (samples 1, 4, 8, 11, 12, and 15) demonstrated the capacity to produce soluble cellulose. A. xylinum (model strain 2) was cultured under static conditions in HS media, demonstrating remarkable efficacy for cellulose sheet production. Subsequent characterization employing scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) unveiled a nano-fiber mat featuring multi-layered fibers. This eco-friendly approach has the potential for large-scale, high-quality cellulose production, applicable in biomedical and industrial fields. The research highlights an environmentally sustainable and economically viable method for cellulose production, presenting potential applicability across biomedical and industrial arenas on a significant scale.

Keywords

Acetobacter xylinum, Bacterial Cellulose, Rotten Fruits, Bacterial Identification, 16SrRNA Sequencing, Multi-layered Fibers

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.