ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Shweta Sagar Naik1 , W. Richard Thilagaraj2, Pooja Gangadharan2 and K.V. Leela1
1Department of Microbiology, SRM MCH RC, Kattankulathur, Tamil Nadu, India.
2Department of Biotechnology, School of Bioengineering, SRM IST, Tamil Nadu, India.
Article Number: 8172 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):401-409.
Received: 17 October 2022 | Accepted: 22 December 2023 | Published online: 24 February 2024
Issue online: March 2024

In recent years, the rapid growth in Antimicrobial resistance (AMR) has become a global concern. Essential oils derived from plants that include bioactive components with proven antioxidative and antibacterial activities could be a potential solution to arrest this problem. In this study, antibacterial activities of DoTERRA essential oils such as Onguard, Clove, Teatree, Lavender and Eucalyptus were evaluated with Indian essential oils against clinical pathogenic bacteria. The GC-MS study revealed that cineole, terpinene, eucalyptol, and eugenol were the most prevalent bioactive components in these essential oils. The purity of the essential oils was confirmed with zeta potential and white light absorption spectrophotometer and shows that the Zeta potential of all the essential oils ranges from -51.4 to 0 mV. Using agar well diffusion and Micro broth dilution procedures, the antimicrobial activity of essential oils of clove, lavender, tea tree, eucalyptus, and On-Guard (combined) was assessed against several multi-drug-resistant bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oils in aqueous and micellar solutions were determined by Micro broth dilution techniques. The most effective antibacterial essential oils were clove, tea tree, and On guard (a blended essential oil with a predominance of Limonene and Eugenol). The current research could result in development of formulas that contain micelle or colloid suspensions of whole essential oils such as clove, On-Guard, or Tea-Tree oil to aid in antimicrobial treatment.


Agar Well Diffusion, Antibiotic Resistance, Essential Oils, Micro Broth Diffusion, Pathogens

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.