ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Riya Dutta1 and Debdulal Banerjee1,2
1Centre for Life Sciences, Vidyasagar University, Midnapore, West Bengal, India.
2Department of Botany and Forestry, Vidyasagar University, Midnapore, West Bengal, India.
Article Number: 9143 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):332-346. https://doi.org/10.22207/JPAM.18.1.17
Received: 18 November 2023 | Accepted: 13 January 2024 | Published online: 19 February 2024
Issue online: March 2024
Abstract

Endophytes are the beneficial group of plant symbionts and are potent sources for producing novel metabolites with biotechnological applications. They are efficient sources for the synthesis of plant growth-promoting substances- Indole acetic acid, ammonia, solubilizing phosphates and thus open up sustainable agricultural opportunities. In the present investigation, twenty-two fungal endophytes were isolated from an ethnomedicinal plant-Helicteres isora L., collected from forests of Singbhum, Jhargram, India, and the isolate-Colletotrichum sp. HelS1, exhibited the most potent in vitro plant growth-promoting activities among all. HelS1 synthesized auxin (111.13 µg ml-1), ammonia, and solubilized phosphate (47.22 µg ml-1) in specific culture conditions. A treatment with fungal extract effectively increases the growth of the experimental plant (tomato seedlings) in terms of improvement in relative water contents, dry weight, nodal length, and pigment profiles compared to the untreated ones. There was an increase in root length by 27% compared to the control group. The isolate produced the maximum IAA after 7 days of incubation on Czepak Dox Broth supplemented with tryptophan (5 mg mL-1), sucrose (30 g L-1), and NaNO(2.2 g L-1) at pH 6.2. Fourier Transform Infrared Spectroscopic analysis of the crude fungal extract further confirmed the production of indole-like compounds. This investigation suggested that secondary metabolites of endophytes act as a potent plant growth inducer and can be utilized in bio-based crop management techniques.

Keywords

Helicteres isoraColletotrichum sp., Growth-promoting Activities

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