ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
K.B. Dhameliya1 , Chetan Ambasana2 and Gaurav Agrawal3
1RK University, School of Science, Environmental Science, Rajkot, Gujarat, India.
2Department of Microbiology, Government Science College, Sector-15, Gandhinagar, Gujarat, India.
3Parul University, School of Engineering, Vadodara, Gujarat, India.
Article Number: 8804 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):280-296. https://doi.org/10.22207/JPAM.18.1.12
Received: 24 June 2023 | Accepted: 05 January 2024 | Published online: 19 February 2024
Issue online: March 2024
Abstract

This study was conducted to isolate and identify bacteria from the wastewater of the textile printing industry inoculated with a mixture of cow dung, jaggery, and urea to assess the bioremediation potential of isolated bacterial species for reducing color and other polluting parameters of the wastewater. Based on colony characteristics, we were able to isolate nine types of bacteria (Pri 1 to 9), capable of thriving in textile printing wastewater. Out of the nine isolates examined, four (Pri 3, Pri 4, Pri 8, and Pri 9) significantly reduced the color and values of other physicochemical parameters of the wastewater. Isolated bacterial cultures were identified using various biochemical tests, antibiotic sensitivity tests, and rRNA sequence analysis. At the end of a 24-h incubation period at room temperature under shaking conditions at 100 rpm on an orbital shaker, isolate Pri 3 was identified as Alcaligenes aquatilis LMG 22996 (T), capable of reducing color by 86.13%, biological oxygen demand (BOD) by 70.44%, chemical oxygen demand (COD) by 80.65%, total dissolved solids (TDS) by 47.31%, total suspended solids (TSS) by 56.56%, and ammoniacal nitrogen by 75.95%; isolate Pri 4 as Priestia aryabhattai B8W22(T), capable of reducing color by 78.35%, BOD by 66.35%, COD by 74.92%, TDS by 34.94%, TSS by 31.66%, and ammoniacal nitrogen by 71.14%; isolate Pri 8 as Citrobacter werkmanii NBRC 105721(T), capable of reducing color by 90.37%, BOD by 82.13%, COD by 85.06%, TDS by 54.83%, TSS by 61.97%, and ammoniacal nitrogen by 80.76%; and isolate Pri 9 as Shewanella chilikensis JC5(T), capable of reducing color by 90.17%, BOD by 84.68%, COD by 83.46%, TDS by 59.13%, TSS by 62.45%, and ammoniacal nitrogen by 90.37%.

Keywords

Textile Printing Wastewater, Cow Dung, Bacterial Isolates, Bioremediation, Physicochemical Parameters, Antibiotic Sensitivity, 16S rRNA Sequencing

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.