ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sherifa A. Saidan1, Raja Jarboui1,2 , Salam S. Alsharari1 and Mona S. Azab1,3
1Department of Biology, College of Science, Jouf University, Saudi Arabia.
2Laboratory of Environment Sciences and Sustainable Development, Sfax University, Preparatory Institute of Engineering Studies of Sfax, BP 1172 – 3018 Sfax, Tunisia.
3Department of Zoology, Faculty of Science, Benha University, Benha 13518, Egypt.
Article Number: 9046 | © The Author(s). 2024
J Pure Appl Microbiol. 2024;18(1):243-256. https://doi.org/10.22207/JPAM.18.1.07
Received: 09 October 2023 | Accepted: 01 January 2024 | Published online: 31 January 2024
Issue online: March 2024
Abstract

This study aims to investigate the morphological and biochemical characteristics of thermophilic bacteria isolated from thermophilic biotopes in the Al-Jouf region of Saudi Arabia for the first time. Additionally, the production of thermostable bacterial enzymes (proteases, amylases, cellulases, gelatinases, and lipases) was investigated. Six main bacterial species were identified via 16S rRNA sequencing and phylogenetic analysis. Twenty water and soil samples were collected from several thermophilic sites in the Dumat Al-Jandal and Al-Qurayyat cities. In total, 28 bacterial strains were isolated and biochemically characterized. Most isolated strains showed high protease and amylase production. 46 and 28% of isolated strains showed the production of cellulase and gelatinase, respectively. However, lipase production was not observed in any isolated bacteria. Four Bacillus licheniformis and two Bacillus subtilis strains exhibited high GC content (55%). Our findings suggest thermophilic bacteria as a source of thermostable enzymes for pharmaceutical and industrial applications.

Keywords

Hot Springs, Salt Marsh Soil, Bacillus licheniformis, Bacillus subtilis, Thermostable Enzymes

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© The Author(s) 2024. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.