ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nenni Dwi Aprianti Lubis1 , Sri Amelia2, Era Yusraini3, Zikrina Rahmi4 and Ridwan Balatif5
1Department of Nutrition, Faculty of Medicine, Universitas Sumatera Utara, Indonesia.
2Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Indonesia.
3Food Science and Technology Department, Faculty of Agriculture, Universitas Sumatera Utara, Indonesia.
4Cell Culture and Tissue Laboratory, Universitas Sumatera Utara, Indonesia.
5Medical Professional Education, Faculty of Medicine, Universitas Sumatera Utara, Indonesia.
Article Number: 8931 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2431-2436.
Received: 23 August 2023 | Accepted: 07 November 2023 | Published online: 29 November 2023
Issue online: December 2023

Aeromonas bacteria are often found in aquatic environments and can be isolated from various types of fish. Globally, the incidence of infection by Aeromonas reached 0.6–76 cases per 1 million people. Orange and lime are generally used as cooking spices to get rid of the fishy smell in fish, and also have antibacterial effects. The study compared the antibacterial effectiveness of lime and orange extracts on two Aeromonas species; A. sobria and A. hydrophila. Bacteria Aeromonas may emerge from fresh tuna bought in traditional markets. Lime and orange extracts were prepared using the maceration method using 96% methanol as solvent. The extract concentrations used were 6.25%, 12.5%, 25%, and 50%. Antimicrobial sensitivity test was carried out using the Kirby-Bauer method.  The bacterial inhibition zone test revealed that the effectiveness of extracts on both oranges and limes was higher for the bacteria A. hydrophila compared to A. sobria. It is known from the average diameter of the inhibition zone, which is larger on A. hydrophila compared to A. sobria, except for the extract with a concentration of 6.25% orange and 50% lime. In a comparison between extracts, lime extract was found to be more effective as an antimicrobial than orange extract, except at a concentration of 6.5% in the bacterial test A. sobria and 50% concentration in the bacterial test A. hydrophila. In general, lime is more effective as an antimicrobial than orange.


Aeromonas sobria, Aeromonas hydrophila, Antimicrobial, Orange, Lime Extract

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.