ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Chammakalayil Sukumaran Arun1, Pambayan Ulagan Mahalingam1 , Haris Parengal2 and Joby Thomas3
1Department of Biology, The Gandhigram Rural Institute (Deemed to be University),
Gandhigram, Dindigul, Tamil Nadu, India.
2Department of Microbiology and Molecular Science, Centre for Tropical Biodiversity Conservation, Ottathara, Kodur, Malappuram, Kerala, India.
3CARe-KERALAM Ltd. KINFRA Small Industries Park, Koratty, Thrissur, Kerala, India.
Article Number: 8972 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2386-2401. https://doi.org/10.22207/JPAM.17.4.33
Received: 06 September 2023 | Accepted: 26 October 2023 | Published online: 29 November 2023
Issue online: December 2023
Abstract

Aflatoxins (AFs) are the most potent and ubiquitously found mycotoxins, capable of causing contamination in agricultural products. Aflatoxin B1 (AFB1) is the most toxic and primarily produced Aflatoxin and will be a real threat to the safety of food and feeds. The current study searched for the potential of Lactic acid bacteria (LAB) isolated from animal excreta for AFB1 mitigation. Three LAB out of 56 isolates were found to exhibit more than 50% sorbent action with AFB1 in phosphate-buffered saline (PBS) and were identified as Lactococcuslactis strain CF_6 (OP183481) (65.38%), Lacticaseibacillus casei strain CW_3 (OP183482)(52.63%) and Lactobacillus acidophilus strain CE_4 (OP183483)(63.13%). More than 60% of the total AFB1 removal was observed in 2 hr of incubation, and maximum sorbent action was found at a pH 6-7 range at 37oC for 24 hours. In the Scanning Electron Microscope (SEM) analysis, heat-killed cells showed a significant increase in cell surface binding area, which improved the surface binding for all isolates except L. casei strain CW_3; however, it proves that LAB surface binding is strain-specific rather than heat treatment. Moreover, the rise in AFB1 concentration improved the rate of the sorbent action but did not observe any substantial changes in total AFB1 detoxification. So, it is concluded that the animal excreta may be a versatile source of probiotic LAB for AFB1 detoxification by surface binding.

Keywords

Aflatoxin B1, Lactic Acid Bacteria, Carcinogen, Sorbent Action, Food Safety, Mycotoxin

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.