ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S. Sri Manjusha Varshini1 , M. Mekala2 and R. Ragunathan3
1Department of Microbiology, Sri Ramakrishna College of Arts and Science for Women, Coimbatore, Tamil Nadu, India.
2Micro Biotek, Coimbatore, Tamil Nadu, India.
3Centre for Bioscience and Nanoscience Research, Coimbatore, Tamil Nadu, India.
Article Number: 8874 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2367-2385. https://doi.org/10.22207/JPAM.17.4.32
Received: 28 July 2023 | Accepted: 20 October 2023 | Published online: 29 November 2023
Issue online: December 2023
Abstract

Acetic acid bacteria synthesized microbial cellulose were isolated from various citrus fruits, enabled by increased cellulose production to develop a biodegradable polymer as a food wrapper. The objective of the research cynosures on the isolation, enrichment, identification, and optimization of bacteria that produce cellulose, characterization, and cytotoxic study of the obtained cellulose. Two highly effective cellulose producers, Acetobacter lovaniensis (A1) and Acetobacter fabarum (A2), were isolated based on their morphology, biochemical analysis, and 16s rRNA sequencing. Studies were conducted to optimize pH, temperature, inoculum size, nitrogen, and carbon sources. Strain A1 produced 0.715 g/100 ml, whereas A2 produced 0.856 g/100 ml of cellulose under optimum growth conditions. The characteristics of microbial cellulose were examined using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). A cytotoxicity study for the obtained cellulose has been conducted with mouse embryo fibroblast cells (3T3-L1) and showed 97% viability of cells with the lowest concentration of 12.5 µg/ml. These isolates could be employed in fermentation technology to produce cellulose polymer-based sustainable biodegradable food wrappers.

Keywords

Acetic Acid Bacteria, Cellulose, SEM, FTIR, 16s rRNA, Cytotoxicity

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.