ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ajad Patel, Ranjan Singh and Rajeeva Gaur
Department of Microbiology, Faculty of Science, Dr. Ram Manohar Lohia Avadh University, Ayodhya, Uttar Pradesh, India.
Article Number: 8897 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2295-2306. https://doi.org/10.22207/JPAM.17.4.26
Received: 06 August 2023 | Accepted: 16 October 2023 | Published online: 20 November 2023
Issue online: December 2023
Abstract

As sugarcane molasses is converted into ethanol, a sizable volume of effluent with high biochemical oxygen demand (BOD) and chemical oxygen demand(COD) is generated. This effluent contains melanoidin. Melanodin is a chemical broken down by certain bacteria and can produce peroxidases, phenoxidases, laccases, and mono- and dioxygenases. The primary function of these bacteria is to break down complex hydrocarbons, including aromatics such as coloring pigments. This study aimed to identify melanoidin-decolorizing microorganisms in natural resources that are thermally resistant and have the potential to be used in industrial-scale distillery treatment for effluent applications. A total of 55 distinct isolates were tested on a solid medium, including molasses pigments. Three thermotolerant bacterial isolates were identified as melanoidin-decolorizing agents: Bacillus nitratireducens (B2), Bacillus paramycoides (B1), and Brucellatritici (B3). These isolates under went additional optimization for decolorization under various physicochemical and nutritional conditions. At 40°C, B. nitratireducens (B2) exhibited the highest degree of decolorization (86%) among the three species while using 0.5% glucose(w/v), 0.5% peptone(w/v), 0.05% MgSO4, and 0.01% KH2PO4 at a pH of 6.0 over 40 h of incubation under static conditions. In submerged fermentation, the B2 strain of B.nitratireducens can withstand higher temperatures and requires only a small amount of carbon (0.5%, [w/v]) and nitrogen sources (0.5%, [w/v]). Therefore, it is feasible to use melanoidin on an industrial scale to decolorize distilled effluents.

Keywords

Melanoidin, Spent Wash, Distillery Effluent, Physico-chemical

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.