ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sarah Benouagueni1 , Asma Labid2, Aida Mehainaoui3 and Djamila Gacemi Kirane3
1Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar-Annaba University, Annaba 23000, Algeria.
2Research Laboratory of Interactions, Biodiversity, Ecosystems, and Biotechnology, Department of Nature and Life Sciences, University 20 August 1955, Skikda, Algeria.
3Department of Biochemistry, Faculty of Science, University Badji Mokhtar Annaba, 23000, Algeria.
Article Number: 8590 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2250-2255. https://doi.org/10.22207/JPAM.17.4.22
Received: 29 March 2023 | Accepted: 05 October 2023 | Published online: 10 November 2023
Issue online: December 2023
Abstract

The overall aim of this paper was to divulge the pathogenic and beneficial bacterial flora of cockroaches (Blatta orientalis) living in a hospital area in Annaba city, Algeria. Thirteen cockroaches were randomly apprehended in sterile conditions during March 2022 in two different hospitals in Annaba, “El Bouni” Hospital and the “Sainte-Therese” Hospital. Bacterial strains were isolated from the imprint of the insect on Petri dishes previously inoculated with different media, also from external and internal body part suspensions of the captured specimens. Biochemical identification was established using the analytical profile Index (API) System (Biomerieux, France). The identification of the enteric-screened strain was confirmed using molecular sequencing of the 16S rRNA gene and phylogenetic analysis was performed. The results showed a high prevalence of the pathogenic strains isolated from the oriental cockroach Blatta orientalis (225 strains), such as Serratia liquefaciens, Raoultella ornithinolytica, Pseudomonas luteola, Enterobacter aerogenes, Hafniaalvei and Bacillus sp. Phylogenetic analysis of the enteric bacteria confirmed the affiliation with Shimwellia blattae NCTC10965 (100%), Basonym Escherichia blattae, which is a natural cyanocobalamin producer. Results confirm at the same time the symbiotic relationship between S. blattae and Blatta orientalis, but also point out the underrepresented potential of these insects as a source of strains with biotechnological interest.

Keywords

Cockroaches, Blatta orientalis, Molecular Identification, Shimwellia blattae, Cyanocobalamin

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.