ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Wafaa Odeh1, Mais Sweiss2 , Fatima Haj Ahmad2, Zeinab Arabeyyat3, Waed Alnsour2, Marah Aldabbas2 and Maen Hasan4
1Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Al-Salt, 19117, Jordan.
2Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt, 19117, Jordan.
3Department of Marine Biology, Faculty of Basic and Marine Sciences, University of Jordan- Aqaba branch, Aqaba, 77111, Jordan.
4Department of Plant Production and Protection, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt, 19117, Jordan.
Article Number: 8921 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2205-2214. https://doi.org/10.22207/JPAM.17.4.17
Received: 16 August 2023 | Accepted: 04 October 2023 | Published online: 01 November 2023
Issue online: December 2023
Abstract

In light of the rapid and extreme changes in climate and the steady depletion of natural resources, there is an urgent need to find innovative and sustainable solutions to these problems. Microorganisms such as microalgae can offer viable solutions to these challenges. Proper investment in such organisms requires the identification of the algal species that inhabit the region. Therefore, this study aimed to isolate and molecularly characterize green microalgae that inhabit freshwater at different locations in the governorates of Irbid and Ajloun in the northern region of Jordan. Water samples collected from these regions were used to isolate single colonies, some of which exhibited different morphological characteristics. Genomic DNA was extracted from the isolates and used as a template for PCR amplification of the 18S ribosomal DNA gene (18S rDNA) and the internal transcribed spacer (ITS) region. Phylogenetic trees were constructed based on 18S rDNA and ITS PCR product sequences, which were used to identify the isolates at the genus level. The obtained isolates belonged to three genera; Coelastrella, Desmodesmus, and Monoraphidium. The latter species has not been previously reported in Jordan.

Keywords

18S rDNA, ITS, Barcoding, Phylogenetic Tree

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.