ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Noelle Christelledes Santos1, Ma. Eloisa Butiong Cabrera1, Janry Durano Flores1, Raymundo Fajardo Javier1, Oliver Redondo Alaijos1, Analiza Joson Molina2 and Christian Joseph Navarro Ong3
1Department of Biology, College of Science, Bulacan State University, Malolos, Bulacan, Philippines.
2Department of Natural Sciences, Angeles University Foundation Integrated School, Angeles City, Pampanga, Philippines.
3Department of Biology, College of Science, De La Salle University, Manila, Philippines.
Article Number: 8749 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2119-2130. https://doi.org/10.22207/JPAM.17.4.08
Received: 05 June 2023 | Accepted: 11 September 2023 | Published online: 23 October 2023
Issue online: December 2023
Abstract

Bioluminescent bacteria (BLB) are the most widely distributed light-emitting microorganisms, most of which are found as symbionts of free-living marine organisms, including the crustacean species. In this study, 4 out of 7 marine crustaceans in the northeastern section of Manila Bay were found to contain bioluminescent bacteria. Marine crustacean species namely Thenus orientalis (Oriental Slipper Lobster), Oratosquilla oratoria (Mantis Shrimp), Penaeus monodon (Giant Tiger Prawn), Litopenaeus vannamei (Whiteleg shrimp), Scylla serrata (Mud Crab), Portunus pelagicus (Blue Swimming Crab), and Charybdis feriata (Crucifix crab), were swabbed after collection. Bioluminescent bacteria were isolated from the inner surface of the carapace, eyes, abdomen, and abdominal segments of the crustacean samples. All glowing colonies were isolated and upscaled. Only bioluminescent bacteria from T. orientalis, O. oratoria, and P. monodon completed the isolation process and were identified using 16S rRNA gene sequencing for phylogenetic analysis. Furthermore, results from molecular identification through Nucleotide BLAST identified that it was Vibrio alginolyticus and Mucus bacterium. At the same time, the presence of Vibrio rumoiensis was also identified which was proved to be understudied and needs exploration. This study was aimed to identify the present bioluminescent bacteria in selected marine crustaceans in Manila Bay, Philippines through microbiological isolation, molecular identification, and phylogenetic reconstruction.

Keywords

Vibrio alginolyticus, Vibrio rumoiensis, Marine Crustaceans, Manila Bay, 16S rRNA Gene Phylogeny

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.