ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Sulakshana Sony Cheemala1 , Aarthi Vara2, M. Swarajya Lakshmi2Shilpa Pradhan2 and K. Kalyani2

1Department of Microbiology, Government Medical College, Ongole, Andhra Pradesh, India.
2Department of Microbiology, Mamata Academy of Medical Sciences, Bachupally, Hyderabad, Telangana, India.
Article Number: 8736 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(4):2111-2118. https://doi.org/10.22207/JPAM.17.4.07
Received: 29 May 2023 | Accepted: 07 September 2023 | Published online: 13 October 2023
Issue online: December 2023
Abstract

Antimicrobial resistance (AMR) in Gram negative bacteria (GNB) has become a critical health concern across the globe. Unveiling of β-lactamase, extended spectrum β-lactamase (ESBL) and AmpC β-lactamase producing bacteria has led to the development of multidrug-resistant organisms (MDRO’s). Carbapenems are considered to be very effective in morbid infections caused by MDRO’s. Now, the upsurge of carbapenem resistance among GNB is an issue of concern as these infections are very difficult to treat. Rapid and reliable methods to detect these CPO’s in all Microbiology laboratories is essential to streamline the antimicrobial therapy. Accordingly, this study is conducted to determine the enormity of CPO’s among clinical isolates by various phenotypic tests along with differentiation of serine β-lactamases from metallo-β-lactamases. This is a Prospective Cross-sectional study meticulously planned & conducted for a period of one year. Among the 76 suspected Carbapenemase Producing Organisms (CPO’s), 42% were Klebsiella spp. followed by Escherichia coli (25%), Pseudomonas aeruginosa (24%), Citrobacter spp. (5%) and Proteus spp. (4%). Out of the total isolates 82% of the isolates were confirmed as CPO’s by Carba NP test, whereas 93% by mCIM test. 53% of the total isolates tested were Serine-β-lactamase producers and 41% were Metallo-β-lactamase producers. In conclusion, Carba NP test and mCIM in conjunction with eCIM test could be considered as reliable phenotypic diagnostic methods for carbapenemase detection to guide the clinicians for initiating antibiotic therapy.

Keywords

mCIM, eCIM, Carba NP, Serine-β-lactamase Producers, Metallo-β-lactamase Producers , Carbapenemase Producing Organisms

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.