ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Muaz Magzob Abdellatif1 , Yahia Hassan Ali1,2 and Hussam Hassan Arafat1,3
1Department of Biology, Faculty of Sciences and Arts, Northern Border University, Arar, Saudi Arabia.
2Virology Department, Central Veterinary Research Laboratory, P.O. Box 8067, Khartoum, Sudan.
3Department of Botany & Microbiology, Faculty of Science, Minia University, Minia City – 61519, Egypt.
Article Number: 8533 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1933-1943.
Received: 28 February 2023 | Accepted: 21 August 2023 | Published online: 03 September 2023
Issue online: September 2023

Brucellosis is a zoonotic disease with veterinary, public health, and economic implications. The study aimed to estimate the seroprevalence of Brucella spp. among ruminants in Rafha, Saudi Arabia during January to October 2022 and to identify camel strains based on a glycosyltransferase gene sequence. Sera (n=1012) were collected from non-vaccinated sheep, goats, camels and cattle of different sex, age and breed randomly from the abattoirs to investigate the circulating brucella antibodies using RBPT. One hundred and eighteen sera (9.7%) were reactive for Brucella spp. IgG immunoglobulins, with higher percentages detected in sheep (11.4%), females (13.3%), adults (10.7%), and naieme breed (13.9%). Significant correlation between Brucella spp. antibodies and animal species (0.095), age (0.077) was found, while strong correlation between antibodies and sex was observed. Glycosyltransferase gene was amplified and sequenced from camel reactive sera (n=6). Camel strains displayed multiple nucleotide substitutions and deletions, nucleotide identity among local strains is 96.2-100%. Phylogenetic analysis showed that Brucella spp. strains clustered in two groups, Rafha strains clustered in one group together with other strains. Further investigation is needed to determine the prevalence of the bacteria among farm animals and to identify the strains involved to improve the preventive measures and strategies adopted for control.


Brucella spp., Seroprevalence, Glycosyltransferase Gene, Ruminants, Rafha, Saudi Arabia

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.