ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Manju1, Amandeep Kaur2, Ashwini Manhas3, Kawal Preet Kaur4, Gundeep Kaur1, Ram Gopal Saini1, Priyanka4 and Manju Singh1
1Centre for Interdisciplinary Biomedical Research, Adesh University, Bathinda, Punjab, India.
2Department of Microbiology Adesh Institute of Medical Sciences & Research, Bathinda, Punjab, India.
3Department of Microbiology, Adesh Medical College & Hospital-Shahbad, Haryana, India.
4Department of Microbiology Maharaja Agrsen Medical College & Hospital, Agroha, Hisar, Haryana, India.
Article Number: 8706 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1887-1892.
Received: 17 May 2023 | Accepted: 10 July 2023 | Published online: 03 September 2023
Issue online: September 2023

In recent years, an increase in Escherichia coli and Klebsiella pneumoniae resistant to carbapenem was reported globally. Due to their high prevalence and extensive range of medical conditions, Escherichia coli and Klebsiella pneumoniae are both confirmed to be major public health concerns. Furthermore, carbapenem resistance restricts treatment options for individuals infected with these bacteria. Consequently, early detection of carbapenem resistance is essential for starting effective therapy, achieving successful management, and avoiding the infection from spreading further in the future. This study’s objective was to identify the phenotypic and genotypic identification of Metallo-β-lactamases (MBL) in carbapenem-resistant E. coli and K. pneumoniae in advanced healthcare facilities. Meropenem resistance was tested in E. coli and K. pneumoniae using the Kirby-Bauer disc diffusion technique. MBL was discovered using a combination of Disc diffusion testing and the Modified Hodge Test. The Polymerase Chain Reaction was used to determine the genotypes of the bla NDM-1 genes that express these enzymes. Out of 427 strains, including 223 E. coli and 204 K. pneumoniae, 35 (8.2%) consisted of carbapenem-resistant, and 29 (82.85%) showed phenotypically verified as metallo-beta-lactamase producers by using the Combined disc test and 20 (57.14%) using the Modified Hodge test. Polymerase Chain Reaction tests for genes detect those three different strains all showed the bla NDM-1 gene. Carbapenemase production and MBL can be recognized with the help of phenotypic combination disc and MHT tests in labs. Since both tests showed 100% concordance, laboratories may use the less expensive CDT instead of the MHT. The current study supports institutional antibiotic stewardship programmes to manage antibiotic use and prevent CRE worldwide.


Carbapenem-resistant, Metallo-β-lactamase, Modified Hodge Test, bla NDM-1, Gene Detection

Article Metrics

Article View: 69

Share This Article

© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.