ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Shubham Sareen1, Afshan Bey1, Afaf Zia1 and Fatima Khan2
1Department of Periodontics and Community Dentistry, Dr. Ziauddin Ahmed Dental College (DRZADC), Aligarh Muslim University (AMU), Aligarh, Uttar Pradesh, India.
2Department of Microbiology, Jawaharlal Nehru Medical college and Hospital (JNMCH), AMU, Aligarh, Uttar Pradesh, India.
Article Number: 8614 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1854-1862. https://doi.org/10.22207/JPAM.17.3.50
Received: 10 April 2023 | Accepted: 21 August 2023 | Published online: 03 September 2023
Issue online: September 2023

The preprint of this article can be found here

Abstract

The study was designed to compare the extent of bacterial colonisation on the surface of Polylactic acid/Polyglycolic acid copolymer and expanded Polytetrafluoroethylene based Guided tissue regeneration (GTR) membrane in an in vitro model by spot analysis and scanning electron microscopy. Earlier in vitro models have aimed to study the barrier function against the bacterial penetration across just one surface of the GTR membranes. No such study is present in the literature which aimed to quantify bacterial adhesion over both the surfaces of the membrane. Sterile Resorbable PLA/PGA copolymer and non-resorbable microporous ePTFE based GTR membrane were used in the study. Both groups were subdivided into two subgroup (n=10) based on incubation period of 24 and 48 hours. Incubated in Todd Hewitt broth with Streptococcus mutans, the samples were vortexed and analysed for bacterial count using spot test and scanning electron microscopy. Between PLA/PGA and ePTFE GTR membrane at 24 hours incubation time period, there was a significant difference in terms of Colony forming units (p = <0.001), with the median Colony forming units being highest in the PLA/PGA GTR membrane. At 48 hours incubation time period, there was a significant difference in terms of Colony forming units (p = <0.001), with the median Colony forming units being highest in the PLA/PGA GTR membrane. Our findings depict that ePTFE based GTR membrane showed significantly lesser bacterial colonisation on its surfaces as compared to PLA/PGA at both the incubation periods i.e., 24 hours and 48 hours as shown by both spot test and SEM.

Keywords

Guided Tissue Regeneration, Bacterial Adhesion, PLA/PGA Membrane, EPTFE Membrane, Spot Test, Scanning Electron Microscopy

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.