ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nur Hasfiana Baktiar1,2, Riuh Wardhani1, Dirayah Rauf Husain1 , Nur Haedar1 and Fuad Gani1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia.
2Pharmacy study program, Sekolah Tinggi Ilmu Kesehatan Maluku Husada, Maluku, Indonesia.
Article Number: 8553 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1791-1799.
Received: 05 March 2023 | Accepted: 08 August 2023 | Published online: 03 September 2023
Issue online: September 2023

Ipomoea pes-caprae is one of the herbal plants that can treat various health problems such as skin infections, burns, boils, and various diseases caused by microbial infections. This study aims to identify ethanol extract compounds of I. pes-caprae leaf and evaluate their antibiofilm activity through in-vitro and in-silico assays. This study used two test bacteria, Pseudomonas aeruginosa and Methicilin Resistant Staphylococcus aureus. Antibacterial activity is carried out using the agar diffusion method and antibiofilm using a microplate reader. The biological activity was also evaluated through a computational approach using molecular docking. The results of preliminary test demonstrated the antibacterial activity. At a concentration of 100 mg/mL,  I. pes-caprae extract produced a substantial inhibitory zone of 13.9 mm for P. aeruginosa and a moderate 8.5 mm zone for MRSA. The extract also showed high antibiofilm activity. It achieved impressive biofilm inhibition rates of 82.58% and 78.29%, respectively. Molecular docking shows the interaction between extract compounds and macromolecules that play a role in biofilm formation, namely SrtA and associated protein biofilms. 1,3,4,5-Tetrahydroxy-Cyclohexanecarboxylic Acid has the lowest binding energy of -7.5 Kcal/Mol and -5.9 Kcal/Mol at each target receptor. This study demonstrated the antibiofilm potential of I. pes-caprae extract, which was clarified through molecular docking studies.


Ipomoea pes-caprae, Biofilm, Antibacterial, In-silico

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.