ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Thi Kim Cuc Nguyen, Thi Lan Pham, Thi Chinh Nguyen, Thu Thuy Pham and Van Duy Nguyen
Institute of Biotechnology and Environment, Nha Trang University, Nha Trang city, Vietnam.
Article Number: 8631 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1605-1611.
Received: 17 April 2023 | Accepted: 08 June 2023 | Published online: 01 September 2023
Issue online: September 2023

The development of genetic technologies and bioengineering are creating an increasing number of genetically engineered microorganisms with new traits for diverse industrial applications such as vaccines, drugs and pollutant degraders. However, the destiny of genetically engineered bacterial spores released into the environment as long-life organisms has remained a big environmental challenge. In this study, an environmentally responsible and sustainable gene technology solution based on the concept of thymine starvation is successfully applied for cloning and expression of a Helicobacter pylori antigen on Bacillus subtilis spore surface. As an example, a recombinant Bacillus subtilis strain A1.13 has been created from a gene fusion of the corresponding N-terminal fragment of spore coat protein CotB in B. subtilis and the entire urease subunit A (UreA) in H. pylori and the fusion showed a high stability of spore surface expression. The outcomes can open the door for developing highly safe spore vectored vaccines against this kind of pathogen and contributing to reduced potential risks of genetically engineered microorganisms released in the environment.


Bacillus subtilis, Genetically Engineered Microorganism, Spore Vectored Vaccine, Thymine Starvation, Urease

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.