ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Anindita Sinha and Keka Sarkar
Department of Microbiology, University of Kalyani, Kalyani, Nadia, West Bengal, India.
Article Number: 8567 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1458-1470.
Received: 13 March 2023 | Accepted: 19 June 2023 | Published online: 20 July 2023
Issue online: September 2023

This study aims to reconcile industrially suitable strains of higher red pigment producing Monascus purpureus MPA5 and higher Monacolin K producing Monascus ruber MRA7, were tried to fuse for development of increased efficacy of targeted secondary metabolites production. The optimum concentration of protoplast mixture was standardized, before the fusion experiment. The effective fusion was observed with the solution of 30 % PEG 6000, CaCl2.2H2O (0.01 M) and glycine (0.05 M). Colonies of the fusants were segregated using complete medium. The fusants were irradiated under UV- light in LD50 dose. On the basis of non-segregation of colony appearance after repeated subculturing in complete medium, 8 colonies were selected. Finally, one of the stable fusant MF11 grew more rapidly than one of the parental strain M. purpureus MPA5, but not as fast as M. ruber MRA7, were selected and grown on low grade rice as solid substrate for production of metabolites. Production of red pigments, alpha-amylase and acid protease were at intermediate stage between two parental strains but enhancement of Monacolin K was observed. High performance liquid chromatography (HPLC) analysis was revealed that Monacolin K content was increased as much as two and half times than the parents. No fluorescent yellow band was detected in TLC plate, indicating of citrinin negativity of the fusant MF11. It is the first report of enhanced Monacolin K production by a stable fusant of Monascus sp.


Protoplast fusion, Monascus purpureus, Monascus ruber, Monacolin K

Article Metrics

Article View: 203

Share This Article

© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.