ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sri Amelia1 , Muhammad Fakhrur Rozi2 and Ridwan Balatif3
1Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.
2Department of Internal Medicine, Faculty of Medicine Universitas Gadjah Mada, Daerah Istimewa Yogyakarta, Indonesia.
3Faculty of Medicine Universitas Sumatera Utara, Medan, Indonesia.
Article Number: 8444 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1429-1434. https://doi.org/10.22207/JPAM.17.3.01
Received: 21 January 2023 | Accepted: 24 May 2023 | Published online: 14 June 2023
Issue online: September 2023
Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) can cause infection with higher morbidity and mortality. In the limited-resource setting, the availability of rapid detection is scarce and may not be available; therefore, prompt detection using the alternative method is indispensable. To compare the detection rate of the modified-conventional method with chromogenic media against mucocutaneous clinical swab, a total of 80 S. aureus isolates from previous studies were cultivated and re-cultured into routine media such as blood agar (BA) and mannitol-salt agar (MSA) between June and September 2018. It directly inoculated from plain blood agar that had been incubated previous day before; it further underwent inoculation to other media, such as chromogenic media (CHROMagar), blood agar and mannitol salt agar that had been supplemented with cefoxitin (CFOX) powder manually. The sensitivity MSA-CFOX and BA-CFOX, respectively, was 96.88%. On the other hand, the sensitivity of CHROMagar and MHA-CFOX was 90.62%. The specificity of each MHA-CFOX, MSA-CFOX, and CHROMagar is 87.5% as well as 93.75% for BA-CFOX. The study has demonstrated better performance of modified-conventional method compared to the other media. Hence, the application of modified-media should not be delayed to facilitate the findings of MRSA among hospitalized patients.

Keywords

Antibiotic Resistance, Cefoxitin Based Media, MRSA, Staphylococcus aureus

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