ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S. Thasneem Banu , S. Vinotha, Radhika Katragadda and R. Vanaja
Institute of Microbiology, Madras Medical College, Park Town, Chennai, Tamil Nadu, India.
Article Number: 8212 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):590-596. https://doi.org/10.22207/JPAM.17.1.56
Received: 05 November 2022 | Accepted: 21 February 2023 | Published online: 03 March 2023
Issue online: March 2023
Abstract

Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus. Following infection, antibodies are formed against the spike (S) and nucleocapsid (N) proteins, which are the primary viral antigens of SARS-CoV-2. This study aims to determine the antibody response three weeks post-infection and its persistence. To study antibody responses in COVID-19-positive individuals and to compare the degree of antibody response in symptomatic and asymptomatic individuals. The persistence of the antibody response was also assessed. Adult patients (> 15 years of age) who were diagnosed as COVID-19-positive by RT-PCR, three weeks after swab positivity were tested for total antibody levels against COVID-19 antigens by electrochemiluminescence assay. Out of 226 individuals, 129 were symptomatic and 97 were asymptomatic. Among the 129 symptomatic individuals, 74 exhibited an antibody response, whereas in the asymptomatic individuals, only 10 exhibited an antibody response. The antibody response was found to be significant in symptomatic individuals compared to that in asymptomatic individuals (p < 0.05). All follow-up individuals were seropositive at the end of both 6 and 8 months. Antibodies against SARS-CoV-2 persist for 8 months following infection. Despite the waning of antibodies against the nucleocapsid antigen, there was no complete disappearance of antibodies.

Keywords

COVID-19, RT-PCR, Electrochemiluminescence assay

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.