ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Pendru Raghunath1, Qursheed Sultana1, Rozina Arshi Khan1 , Shobhalatha Paul1 and Mohammed Abdur Rab Ansari2
1Department of Microbiology, Dr. VRK Women’s Medical College, Hyderabad, Telangana, India.
2Deccan College of Medical Sciences, Hyderabad, Telangana, India.
Article Number: 8038 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):543-548. https://doi.org/10.22207/JPAM.17.1.51
Received: 18 August 2022 | Accepted: 22 February 2023 | Published online: 03 March 2023
Issue online: March 2023
Abstract

Helicobacter pylori is main causative agent of acute gastritis and peptic ulcer disease. In certain population, this infection leads to gastric cancers viz., adenocarcinoma, and mucosal-associated lymphoid tissue (MALT) lymphoma. The objective of this study was to comparatively evaluate invasive (RUT) and non-invasive (IgM and IgG ELISA) methods for detection of H. pylori infection among patients visiting a tertiary care hospital. A total of 285 dyspeptic patients undergoing endoscopic examination were included in this study. From each patient one biopsy specimen and serum samples were collected. Biopsy specimen was subjected to RUT and IgM & IgG ELISA tests were performed using serum samples. H. pylori was detected in 127 (44.6%) and 126 (44.2%) cases by RUT and IgM ELISA, respectively. H. pylori were detected in 85 (29.8%) samples by IgG ELISA. Based on the combination of RUT and IgM ELISA test, total 128 (44.9%) patients were positive for H. pylori infection. Most of the positive cases belonged to 21-40 years age group (60 of 128) followed by 41-60 years age group (31 0f 128). All the three diagnostic methods viz., RUT, IgM ELISA and IgG ELISA used in this study showed a greater prevalence of H. pylori infection in female gender compared to male gender. In this study, sensitivity of both RUT and IgM ELISA was similar across different age groups and gender. The advantage of IgM ELISA over RUT is that it does not require endoscopy. Therefore, IgM ELISA could be considered as safe and an alternative method for detection of this pathogen.

Keywords

H. pylori, Detection, RUT, ELISA

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.