ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Khaled M. Aboelsuod1 , Fatma Sonbol2, Tarek El-Banna2 and Abdelaziz Elgaml1,3
1Department of Microbiology and Immunology, Faculty of Pharmacy, Horus University, New Damietta 34518, Egypt.
2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt.
3Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Article Number: 8387 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):486-498. https://doi.org/10.22207/JPAM.17.1.42
Received: 28 December 2022 | Accepted: 16 February 2023 | Published online: 02 March 2023
Issue online: March 2023
Abstract

Pseudomonas aeruginosa is an expedient Gram-negative bacterium, which is characterized by its ability to acquire antimicrobial resistance. In this study, 56 unrepeatable carbapenem-resistant P. aeruginosa isolates were gathered from various clinical sources from hospitals in Cairo and Mansoura universities. The isolates exhibited diminished susceptibility towards carbapenems, quinolones, aminoglycosides and chloramphenicol by using disc diffusion method. Carbapenemase production was confirmed among the isolates, where all the 56 P. aeruginosa isolates harboured carbapenemase genes including blaVIM (43 isolates), blaKPC (38 isolates), blaNDM-1 (17 isolates), blaIMP (16 isolates) and blaOXA-48 (15 isolates). Among the isolates, 13 carried only one carbapenemase gene, while 43 isolates carried multiple carbapenemase genes. MCR-1 production was confirmed in 10 of the tested isolates by detecting the mcr-1 gene encoding for the colistin resistance. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) evaluation showed that the tested isolates were unrelated to each other. Therefore, this study rises the danger of emergence of MDR P. aeruginosa resistant to carbapenems coupled with other antimicrobials including colistin, which is regarded as the last reservoir for the management of infections caused by MDR Gram-negative pathogens. Early inspection of resistance patterns in MDR organisms is an important tool to control and prevent infections via limiting the spread of these pathogens.

Keywords

Carbapenemase, Colistin, Egypt, Multidrug-resistance, Pseudomonas aeruginosa

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.