ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ibrahim A. Abdelwahab1 , Rania R. Abozahra2, Shawky A. Sultan3, Hala M. Abd-elaal4 and Sarah M. Abdelhamid2
1Department of Microbiology and Immunology, Faculty of Pharmacy, Pharos University in Alexandria, P.O. Box 37 Sidi Gaber, Alexandria, 21311, Egypt.
2Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour University, Damanhour 22511, Egypt.
3Ministry of Health and Population, Cairo 11516, Egypt.
4Hematology Department, Damanhour Medical National Institute, Damanhour 22511, Egypt.
Article Number: 8326 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):467-485. https://doi.org/10.22207/JPAM.17.1.39
Received: 13 December 2022 | Accepted: 11 February 2023 | Published online: 02 March 2023
Issue online: March 2023
Abstract

Microbial interactions are frequently categorized according to how they affect each population in a binary system. We aimed to determine the interaction between P . aeruginosa, S . aureus, and E . coli in-vitro. In this experimental hospitalized patients’ sputum, urine, and blood samples were used to collect a total of 90 clinical isolates for the study in Damanhour Medical National Institute, Behira, Egypt, followed by accurate identification and testing for antibiotic sensitivity. To examine the effect of the supernatant of P. aeruginosa on S. aureus and E. coli determined MIC using broth microdilution method. We also measured the activity of lasA protease by assessing the S. aureus cell lysis potential of P. aeruginosa culture supernatants. Extraction of pyocyanin was made to determine the change in the cell nature of S. aureus upon exposure to pyocyanin by using a scanning electron microscope and the shape of colonies on the culture media was determined. Finally, we detect lasA, operon phz, phzM, phzS and rhlAB genes for P. aeruginosa. P. aeruginosa showed a great impact on S. aureus isolates resistant to different antibiotics as it facilitates their killing and may drive the normal colonies of S. aureus into SCVs. The ability to form biofilm by S. aureus and E. coli decreased in the presence of Pseudomonas supernatant.

Keywords

Biofilm, Microbial Interactions, P. aeruginosa Interactions, E.coli Interactions, S. aureus Interactions, Operon phz

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.