ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ajit Pratap Singh Yadav1, Vinay Dwivedi2 , Satyendra Kumar3 and Anil Kumar4
1Department of Biotechnology, Rama Institute of Engineering and Technology, Kanpur, Uttar Pradesh, India.
2Department of Biotechnology, Engineering and Food technology, Chandigarh University, Chandigarh, India.
3Department of Botany, B.N.M.V. College, B.N.M. University, Madhepura, Bihar, India.
4Central Research Laboratory, Rama Medical College Hospital and Research Centre, Mandhana, Kanpur, Uttar Pradesh, India.
Article Number: 8173 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):439-448. https://doi.org/10.22207/JPAM.17.1.36
Received: 17 October 2022 | Accepted: 09 February 2023 | Published online: 02 March 2023
Issue online: March 2023
Abstract

Chromium (VI) is a well-known pollutant that is present in industrially polluted soil and water, and has been reported to be mutagenic and carcinogenic. In the present study, we investigated the effective use of Leptolyngbya boryana (cyanobacterium) as an eco-friendly option to overcome Cr (VI) toxicity in tannery effluents. The main objective of this study was to identify the Cr reductase (ChrR) gene and its variability in the context of Cr (VI) stress. Industrial polluted soil samples were collected and processed according to standard protocols for ChrR variation and 16S rDNA gene analysis. Genomic DNA was isolated from the collected samples and the ChrR and 16S rDNA genes were amplified by PCR. Amplified 16S rDNA was sequenced and aligned with known sequences. In the present study, a strong correlation was established between the nucleotide sequences of the ChrR and 16S rDNA genes. The Minimum Inhibitory Concentration (MIC) was determined for Cr (VI), and pure strains of L. boryana were identified and isolated from soil samples. Cr (VI)-stressed conditions and their genetic variability were confirmed by sequencing. In conclusion, the L. boryana strain has been identified an eco-friendly option for overcoming Cr (VI) toxicity in tannery effluents.

Keywords

Bioremediation, ChrR gene, Leptolyngbya Boryana, Genetic Variability, Chromium Reductase, Homology

Article Metrics

Article View: 633

Share This Article

© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.