ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ram Bahadur Khadka1 , Balram Neupane1, Gautam Prasad Chaudhary1, Khimdhoj Karki1, Arjun Prasad Pokhrel1, Dhakaraj Pant2, Santosh Kumar Gupta2, Jitendra Pandey3 and Rabin Gyawali4
1Department of Health Science, Crimson College of Technology, Pokhara University, Nepal.
2School of Health & Allied Sciences, Pokhara University, Nepal.
3Department of Chemistry, 2545 McCarthy Mall, University of Hawai’i at Manoa, Honolulu, HI 96822, USA.
4Rapti Life Care Hospital & Nursing College, Dang, Nepal.
Article Number: 7743 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):380-384.
Received: 07 April 2022 | Accepted: 02 February 2023 | Published online: 01 March 2023
Issue online: March 2023

Dengue virus (DENV) is spread mostly by biting by the infected female mosquitoes of the species Aedes aegypti, that are commonly found in tropical and subtropical zones of globe. Some studies have shown that nearly fifty percent of the globe are susceptible to DENV. Most infections by DENV are asymptomatic. Clinical infections vary from mild fever to serious diseases including hemorrhagic fever and shock syndrome. The main objective of this study was to estimate DENV infection by rapid diagnostic (RDTs) kit methods in a tertiary care setting. This retrospective study was done at Crimson Hospital, Butwal, Nepal from June to November 2019. Serum samples of all dengue fever suspected patients were tested for DENV antigen as Dengue nonstructural protein 1(NS1) and DENV specific antibodies as IgM and IgG by using World Health Organization Good Manufacturing Practices (GMP) certified rapid solid phase immunochromatographic method. All laboratory test results were tabulated by using MS-Excel and analyzed by statistical package for the Social Sciences software version 20. Out of 821 samples tested, 518 were positive, including 322 (62.16%) reactive for dengue NS1 Protein, 121 (23.35%) for IgM, 62 (11.96%) for IgG and 13 (2.50%) were positive for both IgG and IgM antibodies. Our study showed high prevalence of dengue infection during monsoon to post-monsoon and the infection rate was higher in males (71.72%) than in females (53.36%). According to our findings, DENV infection is prevalent in Butwal, Nepal. Early diagnosis, better case management, faster public health response and effective health policy towards the control of mosquito vectors may reduce the dengue burden and must be implemented in Nepal.


Dengue Fever, Immunoglobin G (IgG), Immunoglobin M (IgM), Non-Structural Protein 1 (NS1), RDTs

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.