ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Gottumukkala Hiranmayee, Saravani Alla, Ananya Lakamsami,
Kanumilli Hima Madhuri Devi and Golamari Siva Reddy
Department of Bio-Technology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India.
Article Number: 8139 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):320-328.
Received: 30 September 2022 | Accepted: 04 January 2023 | Published online: 01 March 2023
Issue online: March 2023

The present study focusses on proving the biosurfactant activity of the 7 isolated bacterial strains. Plants could not absorb enough nutrients as a result of contamination in the soil. This contaminants can be chemical pollutants or oil spillage. By considering the earlier potential applications of biosurfactants, this research was aimed to prove the role of a plant probiotic bacteria as oil degrading bacteria. In order to prove this approach, many screening methods were performed such as drop collapse, oil spreading (mm), emulsification stability testing by calculating emulsification index (E24%), penetration assay etc. Among the 7 selected isolates, isolate KL-015 have shown positive results with all the biosurfactant screening analysis. A unique role was proven by KL-011 isolate as it has shown a high oil degrading zone, i.e., 0.6 mm in diameter as well as the formation of more foam layer or emulsified layer with the emulsification index of 17.39% which indicates its role in having a biosurfactant molecule within it.


Biosurfactant, Oil Spillage, Drop Collapse, Oil Spreading, Emulsification Index, Penetration Assay

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.