ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Khadeeja S. Madhi1, Mohammed H. Khudor2 and Rasha M. Othman2
1College of Medicine, University of Basrah, Basrah, Iraq.
2College of Veterinary Medicine, University of Basrah, Basrah, Iraq.
Article Number: 7168 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):289-297.
Received: 04 July 2021 | Accepted: 19 December 2022 | Published online: 03 February 2023
Issue online: March 2023

Antimicrobial activity of certain species of Lactobacillus bacteria were known to produce a certain type of bacteriocin called Plantaricin (Pln). In this study, the PlnF (plnF) gene (666 bp) encoding plantaricin was synthetically constructed based on a sequence of predefined and valuable amino acid compositions and subcloned into pET-28 (+) expression vector. Thereafter transformation of recombinant vector into BL21 (DE3) competent E. coli was achieved and to verify the result of cloning, the PCR colony technique was conducted. The expression of the inserted gene was analyzed by the SDS-polyacrylamide gel electrophoresis method, which detected a band of approximately 30 KD molecular weight for interested protein as a natural bioactive bacteriocin.


Lactobacillus, Cloning, Synthetic Gene, Plantaricin F. Expression, Bacteriocin

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.