ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sri Purwaningsih , Dwi Agustiyani, Tirta Kumala Dewi and Sarjiya Antonius
Research Center for Applied Microbiology, National Research and Innovation Agency, Cibinong, West Java, Indonesia.
Article Number: 7906 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):231-240.
Received: 14 June 2022 | Accepted: 07 December 2022 | Published online: 03 February 2023
Issue online: March 2023

Plant growth-promoting rhizobacteria (PGPR), which were isolated from the plant rhizosphere, decrease the addition of chemical fertilizer (N) and promote plant growth. Some PGPR isolates can fixate nitrogen, solubilize phosphate, produce phytohormones, and control soil pathogens. This study has focused on isolating rhizobacteria from root nodules of Edamame, Glycine max L, Dieng Peanut, Solanum sp. and Peanut root. Eleven isolates were assayed to examine their activities, including the ability of nitrogen fixation, produce Indol Acetic Acid hormone, siderophore, ammonia, and catalase activity. Furthermore, 11 isolates were tested to promote soybean growth in pot experiments using sterile sand media and the test of symbiotic capacity. The results showed that six isolates (RhizE2, RhizE3, RhizE4, RhizKdKbm1, Bio2DW, and Bio3DW) could form root nodules, and four isolates (RhizE2, RhizE3, RhizKdKbm1, and Bio2DW) showed a symbiotic capacity of more than 90%. RhizE2 significantly increased the number of nodules and dry weight of the entire plant (24.08 g) in comparison with plants without inoculation and added N fertilizer (11.59 g) and added N fertilizer (19.60 g). RhizE2 can be further developed as a biological fertilizer agent, especially for soybean plants. From all parameters observed, the plants inoculated with RhizE2 showed the best growth result.


Nitrogen Fixation, Nodulation, Rhizobacteria Soybean, Sterile Sand Medium

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.