ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Shalini Shriwastav1, Narinder Kaur1, Rosy Bala1, Jai Dev1,Mahmudul Hassan2, Harshita3, Chainika Khatana4, Divya Mittal5 , Shahbaz Aman1 and Jyoti Chauhan1
1Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India.
2Department of Microbiology, AIMSR, Adesh University, Bhatinda, Punjab, India.
3Department of Biotechnology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India.
4Central Research Laboratory, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India.
5Department of Microbiology, Shoolini University of Biotechnology and Management Sciences, Bajhol, Himachal Pradesh, India.
Article Number: 8118 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):222-230.
Received: 24 September 2022 | Accepted: 17 December 2022 | Published online: 24 January 2023
Issue online: March 2023

Non-fermenting gram-negative bacteria (NFGNB) endeavouring as major pathogen in infectious disease, predominantly in urinary tract infection (UTI) and increased resistance in NFGNB are matter of concern. This study aimed to evaluate the frequency of NFGNB, antibiotics resistance pattern, plasmid profiling, and antibacterial efficacy of Moringa oleifera against NFGNB. NFGNB were isolated from clinically suspected UTI patients. Identification of isolates and their antibiotics sensitivity pattern were analyzed according to conventional method, and Vitek 2 automated system. Moreover, NFGNB were evaluated for biofilm production and presence of plasmid. Furthermore, antibacterial activity of Moringa oleifera was evaluated against NFGNB. P. aeruginosa (86.0%), and A. baumannii (10.0%) were the most frequent NFGNB followed by Providencia rettgeri 2.0%, Stenotrophomonas maltophilia 1.0%, myroides species 1.0%. 68.6% P. aeruginosa and 60.0% A. baumannii were biofilm producers whereas imipenem and meropenem were the most effective antibiotics. Isolated NFGNB showed multiple bands of plasmid. Furthermore, Moringa oleifera leaves extract showed antibacterial activity against tested NFGNB. MDR-NFGNB presents challenges in treatment and Moringa oleifera leaf extract may be used as an alternative medicine. However, the therapeutic role of specific ingredients present in extract needs further investigation and purification.


Multi-drug Resistant, Uropathogen, Non-fermenting Gram-negative Bacteria, Plasmid Profile Analysis, Moringa oleifera

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.