ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Umakant Banjare1, Arun Kumar Patel3, Ashish Kumar Pandey1, Santosh Kumar1, Ramesh Kumar Singh1, Prahlad Masurkar2, Rakesh Kumar Singh2 and Sandeep Kumar Gupta2

1Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
2Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
3Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Article Number: 7826 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):155-166.
Received: 07 May 2022 | Accepted: 03 December 2022 | Published online: 16 January 2023
Issue online: March 2023

Growth promoting Rhizobium spp are frequently used as biofertilizers for agricultural cropping system. Furthermore, Isolation, screening and biochemical characterization of Rhizobium for a specific plant is necessary to examine ability of isolated bacteria to affect the growth and development of host plant in various ways. The current study was aimed to isolate plant specific rhizobacterial strains which are compatible with lentil (Lens culinaris Medik.L.) plant. 20 bacterial isolates have been isolated from root nodules of lentil from various agro ecological area and their biochemical characterization was performed by different plant growth promotion activities. The result showed that, among 20 isolates, four isolates have vigorous plant growth promoting activities. Four bacterial strains were able to solubilise phosphorous along with hormone production. Moreover, among four bacterial strains, two strongly produced HCN and siderophore in vitro. Subsequently, all selected bacterial isolates were inoculated in lentil seeds of variety HUL57 to study germination percentage and vigour index of the crop. Out of four isolates 26N isolate performed best growth promotion activities on lentil seedlings. Finally, on the basis of performance of bacteria on plant, four isolates were characterized using molecular approach of species identification such as 16S rRNA sequencing.


Lentil, Rhizobium spp, PGPR, 16SrRNA Sequencing, Seed Germination, Vigour Index

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.